1Department of Pharmacology, School of Pharmaceutical Sciences, Lovely Professional University, Jalandhar, Punjab, INDIA
2Department of Pharmaceutics, Azad College of Pharmacy, Peddamangalaram, Moinabad, Hyderabad, Telangana, INDIA
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ABSTRACT
Aim/Background
The aim is to evaluate THMQ’s impact on alcohol withdrawal and alcohol craving using an animal model. Mice were exposed to increasing ethanol doses to simulate dependency. Researchers assessed anxiety levels during withdrawal using behavioural and biochemical tests. THMQ was administered orally at 20 mg/kg and 40 mg/kg, compared to diazepam (1 mg/kg).
Materials and Methods
Model Creation Mice received escalating ethanol doses (5% to 35% v/v) over specific days. Regular water was given during withdrawal periods. Assessment Tools Elevated Plus Maze (EPM), Elevated Zero Maze (EZM), Open Field Test (OFT), and Western Blot analysis. Treatment Intervention: THMQ (20 mg/kg and 40 mg/kg) vs. diazepam (1 mg/kg).
Results and Conclusion
THMQ-treated group showed reduced anxiety and alcohol desire. Alcohol consumption decreased in THMQ-treated mice. No changes in GluA1 and sk2 protein levels. THMQ holds promise as a remedy for alcohol cravings and withdrawal symptoms