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Published on:March 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(2):465-470
Original Article | doi:10.5530/ijper.54.2.53

HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2


Authors and affiliation (s):

Vania Maslarska1, Meri Amerikova2, Ivanka Pencheva2, Stanislav Bozhanov1

1Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav st., 1000 Sofia, BULGARIA.

2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav st., 1000 Sofia, BULGARIA.

Abstract:

Aim: The aim of this research study was to develop a simple, rapid, precise, accurate and economical RP-HPLC method, with a simple mobile phase for the identification and determination of the antimicrobial peptide α-defensin-2. Materials and Methods: Separation was carried out at 25°C, using column Luna 5U (C18, 250x4.6, 5 μm) with mobile phase consisting of acetonitrile: water (40: 60 v/v). The detector was set at 210 nm. The flow rate was 1.2 ml/min and injection volume was 20 μl. Results: The developed method was validated as per ICH guidelines and the kinetic behavior of α-defensin 2 was studied in respect of different pH. Conclusion: The obtained data can serve for creating an in vivo monitoring program and quality control for different studies and preparations.

Key words: Antimicrobial peptides, α-defensin 2, HPLC, Chemical stability, Analysis.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs