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Published on:August 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(3):761-770
Original Article | doi:10.5530/ijper.54.3.128

In vivo Anti-diabetic and Anti-hyperlipidemic Activities of ethyl Acetate/Methanol Fractions of Feronia elephantum Fruit in type 2 Diabetic Rats: Via α-amylase and PPAR-γ by using in silico Approach


Authors and affiliation (s):

Gangavaram Jyothi Reddy1,*, Kesavan Bhaskar Reddy2, Gopireddy Venkata Subba Reddy3

1Department of Pharmaceutical Sciences, JNTUA, Ananthapuramu, Tirupati, Andhra Pradesh, INDIA.

2Sri Venkateswara College of Pharmacy, RVS Nagar, Chittoor, Andhra Pradesh, INDIA.

3Department of Chemistry, JNTUA College of Engineering, Pulivendula, Andhra Pradesh, INDIA.

Abstract:

Background: Obesity and overweight, together with physical inactivity, are projected to cause a huge proportion of the global diabetes burden. Natural products play a key role in the discovery of novel therapeutic agents. Aim: The present study aimed to elucidate in silico and in vivo anti-diabetic and anti-hyperlipidemic potential of Ethyl acetate (EFE) and ethyl acetate:methanol (EMFE) fractions of F. elephantum fruit in diabetic rats. Methods: Male Sprague-Dawley rats were induced type 2 diabetes by high fat diet (HFD) for a period of 2 weeks and then injected intraperitoneally with Streptozotocin (STZ, 35 mg/ kg b.w). Results: Significant antihyperglycemia was displayed in rats treated with EFE and EMFE in OGTT in normal rats. Daily oral treatment with EFE and EMFE each at 100 and 200 mg/kg b.w for 5 weeks resulted in significant reduction in plasma glucose levels and HbA1c, increased plasma insulin levels, normalised lipid profile and liver function parameters in HFD and STZ model. In addition, degenerative histopathological changes were found to be normalized. Molecular docking studies revealed strong binding efficacy of the active constituents of the extract viz., 22,23-dihydrostigmasterol, tocopherol and campesterol with α-amylase enzyme and PPAR-γ. Conclusion: These results suggest a promising anti-diabetic and anti-hyperlipidemic effect of Feronia elephantum fruit without apparent toxic effects.

Key words: Molecular Docking, Feronia elephantum, Anti-diabetic, Anti-hyperlipidemic, Streptozotocin, High Fat Diet.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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