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Published on:August 2016
Indian Journal of Pharmaceutical Education and Research, 2016; 50(3):489-494
Original Article | doi:10.5530/ijper.50.3.24

Development and Validation of a RP-HPLC Method for the Simultaneous Estimation of Sulfadoxine and Pyrimethamine in Combined Dosage Tablets.


Authors and affiliation (s):

Sanjay Pai PN, Cynella Dias and Neelam Sawant

Department of Pharmaceutical Analysis, Goa College of Pharmacy, 18th June Road, Panjim-403001, Goa, INDIA.

Abstract:

A new simple, sensitive and reproducible RP-HPLC method for the simultaneous estimation of sulfadoxine and pyrimethamine in pharmaceutical formulations was developed and validated. The separation was carried out on Zorbax Eclipse Plus C18, 3.5 μm (4.6×100 mm) column with acetonitrile: 20 mM sodium acetate buffer pH 6, 80:20% v/v as the mobile phase at the flow rate of 1 ml/min. The eluent detection was carried out using UV-Visible PDA detector at 224 nm. The retention time of sulfadoxine and pyrimethamine was 0.9 min and 1.2 min respectively. Linearity was observed for sulfadoxine and pyrimethamine in the concentration range of 60-200 μg/ml and 3-10 μg/ml respectively. The recovery of sulfadoxine and pyrimethamine was found to be 97.21-98.56% and 96.90-97.26% respectively.

Key words: RP-HPLC, Sulfadoxine, Pyrimethamine, Validation, Stress-induced stability.

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