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Published on:October 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(3s):s570-s579
Original Article | doi:10.5530/ijper.54.3s.156

In vitro Antioxidant Potential and Cytotoxicity Study of Asparagus aethiopicus L. Extracts on HT-29 Human Colon Cancer Cell Line


Authors and affiliation (s):

Suhas Suresh Awati1,2,*, Ritu M Gilhotra1, Santosh K Singh1, Vinit Raj1, Kiran A Wadkar3

1School of Pharmacy, Suresh Gyanvihar University, Jaipur, Rajasthan, INDIA.

2Dr. Shivajirao Kadam College of Pharmacy, Kasabe Digraj, Sangli, Maharashtra, INDIA.

3Department of Pharmacognosy, Appasaheb Birnale College of Pharmacy, Sangli, Maharashtra, INDIA.

Abstract:

Objectives: To decide the phytochemical constituents, antioxidant and anticancer potential of Asparagus aethiopicus L. extracts on HT-29 human colon malignant growth cell line. Methods: The roots of plant were exposed to Hot Soxhlet continuous extraction with expanding polarity of solvents viz., pet ether, chloroform, ethanol and aqueous maceration. Qualitative phytochemical screening was completed by utilizing distinctive phytochemical tests. The antioxidant potential was tried utilizing 2, 2-diphenyl-1- picrylhydrazyl, ferric ion reducing power assay and phosphomolybdenum assay. In vitro anticancer action tried on HT-29 human colon malignant growth cell line and it was assessed by (3-(4, 5-dimethyl thiazole-2yl)- 2, 5-diphenyl tetrazolium bromide) MTT test. Results and Discussion: Preliminary qualitative phytochemical screening affirmed the presence of phytoconstituents like alkaloids, flavonoids, glycosides, saponins, sterols, tannins and reducing sugar. Antioxidant potential was demonstrated most noteworthy in ethanol extracts dependent on the test performed. The ethanol extracts were seen as an antioxidant and specifically cytotoxic to HT-29 human colon malignant growth cell line. Conclusion: The outcomes show that Asparagus aethiopicus L. having a potential of antioxidant activity and anticancer action on HT-29 human colon cancer cell line. In any case, further examinations are expected to presume that the particular constituent liable for its antioxidant action and canceMethods:r prevention agent.

Key words: Phytochemical, Antioxidant, Anticancer, Colon cancer, Asparagus aethiopicus.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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