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Published on:October 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(3s):s423-s432
Review Article | doi:10.5530/ijper.54.3s.140

Columns in Pharmaceuticals: For Primers

Authors and affiliation (s):

Sanjay Sharma1, Manish Sisodiya1, Rupesh Kumar Gautam2,*, Swapnil Goyal3

1School of Pharmacy and Technology Management, SVKM’SNMIMS, Shirpur, Maharashtra, INDIA.

2Department of Pharmacology, MM. School of Pharmacy, Maharishi Markandeshwar University Sadopur-Ambala, Haryana, INDIA.

3BR Nahata College of Pharmacy, Mandsaur University, Mandsaur, Madhya Pradesh, INDIA.


Chromatographic processes have a crucial role in the manufacturing of pharmaceuticals, bio-pharmaceuticals, polypeptides, proteins, enzymes and antibodies. They are apprehended, purged and elegant by size exclusion, ion exchange, reverse phase, hydrophobic interaction or the affinity chromatographic processes. The key to the proper selection of column is the knowledge of the principles of the chromatographic process (HPLC, Ion exchange, size exclusion, gas chromatography, UPLC). A Chromatographic column is a tool used for the separation of various chemical compounds in mixtures. A chromatographic column comprises the stationary phase, letting the mobile phase to go from it. Chromatography columns of the various classes are used in gas and liquid chromatography. Make certain robustness and reproducible performances of column, columns are needed to be qualified in an acceptable fashion before each use. This article describes the column specification and enables the reader or industrial primers to make the sense of the many parameters in details such as particle size, dimensions, surface area, pore size, carbon load which is used to describe HPLC columns, ion-exchange chromatography columns, size exclusion columns, chiral columns, gas chromatographic columns and UPLC chromatographic columns with application.

Key words: Column, Specifications, HPLC, Size exclusion, Gas chromatographic, UPLC, Bonded Phase.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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