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Published on:December 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(4):1039-1045
Original Article | doi:10.5530/ijper.54.4.198

Asarone and Metformin Modulates the Oxidant- Antioxidant Imbalance on Experimentally Induced Hepatocellular Carcinoma during Diabetic Condition


Authors and affiliation (s):

Bhrigu Kumar Das1,2, Pramod Channabasappa Gadad1,2,*

1Department of Pharmacology, KLE College of Pharmacy (A Constituent Unit of KLE Academy of Higher Education and Research, Belagavi), Vidyanagar, Hubballi, Karnataka, INDIA.

2Off-campus Basic and Applied Sciences Research Centre of KLE Academy of Higher Education and Research at KLE College of Pharmacy, Vidyanagar, Hubballi, Karnataka, INDIA.

Abstract:

Aim: The aim of the present study was planned to investigate the efficacy of asarone and metformin HCl as an antioxidant on experimentally induced diabetic-hepatocellular carcinoma (HCC) condition in male Wistar rats. Methods: Diabetes was induced in experimental rats by single intraperitoneal injection of streptozotocin (STZ; 55 mg/kg b.w.). Following two weeks of STZ injection, the diabetic-HCC condition was simulated in the rats with a single intraperitoneal dose of diethylnitrosamine (DEN; 200 mg/ kg b.w.). The α-and β-asarone combination in the ratio of 1:1 (50 μg/kg b.w.) and metformin HCl (250 mg/kg b.w.) treatment were orally given to the diabetic-HCC rats up to 18-weeks. At the end of the experimental period, rats were sacrificed after the withdrawal of blood for biochemical and liver samples were isolated for the antioxidant and histopathological analysis. Results: The elevated levels of liver function test such as alkaline phosphatase, bilirubin and decreased levels of total protein, albumin and globulin indicated the hepatic damage in the diabetic-HCC rats. Further, the STZ+DEN-induced oxidative stress was confirmed by the elevated levels of lipid peroxidation (LPO) and decreased levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and Vitamin-C. Treatment with asarone and metformin significantly ameliorated the STZ+DEN-induced hepatic damage and oxidative stress. Histopathological evidence also showed recovery of the hepatic architecture in diabetic- HCC rats treated with asarone and metformin. Conclusion: Asarone and metformin exhibit good hepatoprotective and antioxidant potential against STZ+DEN-induced HCC in rats.

Key words: Asarone, Diethylnitrosamine, Metformin, Oxidative stress, Streptozotocin.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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