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Published on:August 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(3):857-862
Original Article | doi:10.5530/ijper.55.3.159

Bacterial Endophyte Inhabiting Durio zibethinus and its Radical Scavenging and Antidiabetic Potential


Authors and affiliation (s):

Sridevi Chigurupati1, Shantini Vijayabalan2, Vasanth Raj Palanimuthu3, Suprava Das4, Saurabh Bhatia5

1Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraidah, SAUDI ARABIA.

2School of Pharmacy, Faculty of Health and Medical Sciences, Taylor’s University, Subang Jaya, Kuala Lumpur, MALAYSIA.

3Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, JSS Academy of Higher Education Research, Ooty, INDIA.

4Department of Pharmacology, Faculty of Medicine, AIMST University, Semeling, 08100 Bedong, Kedah, MALAYSIA.

5Natural & Medical Sciences Research Center, University of Nizwa, P.O. Box 33, 616 Birkat Al Mauz, Nizwa, Oman; Amity Institute of Pharmacy, Amity University Haryana, Gurgaon, INDIA.

Abstract:

Background: Endophytes, markedly receiving interest and has been detected to be remarkable sources of bioactive metabolites. Endophytes, which are also called as endosymbionts have pulled into consideration in the discovery for novel bioactive compounds that can be utilized as new medications. In the current study, endophyte has been isolated from Durio zibethinus leaves. 16s rRNA partial genome sequencing was used to identify selected endophyte and the obtained bacterial endophytic crude extract (DZLM) was subjected for the antioxidant and antidiabetic investigations. Results: Cronobacter sakazakii was identifies as the endophye. Qualitative phytochemical test on DZLM confirmed the presence of phenols, carbohydrates, alkaloids, flavonoids, steroids, mucilage, and glycosides. The antioxidant assay of DZLM showed the good antioxidant potency with IC50±SEM: 161±0.08 μg/mL for DPPH assay and IC50±SEM: 126± 0.09 μg/mL for ABTS assay. Antidiabetic assay results demonstrated a dosedependent percentage inhibition of the enzymes. The results revealed good inhibition of α-amylase with IC50±SEM: 239±0.08μg/mL and α-glucosidase inhibition with IC50±SEM: 241±0.08 μg/mL for DZLM compared to the standard drug, acarbose. Conclusion: The study uncovered the fact that DZLM has a wellspring of natural source of antioxidant and antidiabetic agents and further studies are required for fractionation of the active constituents existing in DZLM and also their structural elucidation which supports the application of endo-symbionts as the basis of future natural medicines.

Key words: Cronobacter sakazakii, Durio zibethinus, Endophytes, α-amylase, α-glucosidase.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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